cd209 dc sign microbead kit (Miltenyi Biotec)
Structured Review

Cd209 Dc Sign Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd209+microbead+kit/CD209+(DC-SIGN)+MicroBead+Kit%2C+human/pmc10217447-166-12-16
Average 91 stars, based on 1 article reviews
Images
1) Product Images from "The Epigenetic Reader Protein SP140 Regulates Dendritic Cell Activation, Maturation and Tolerogenic Potential"
Article Title: The Epigenetic Reader Protein SP140 Regulates Dendritic Cell Activation, Maturation and Tolerogenic Potential
Journal: Current Issues in Molecular Biology
doi: 10.3390/cimb45050269
Figure Legend Snippet: GSK761 impairs DCs maturation. Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. The cells were then kept unstimulated (iDCs) or stimulated (maturation) with 100 ng/mL LPS for 24 h (mDCs). Differentiation and maturation markers were assessed by FACS. ( A ) Data are illustrated as FACS plots or ( B ) percentage of CD11c + , CD14 + , CD80 + , CD83 + , CD86 + and CD209 + cells from total alive cells, n = 3 ( C ) by histogram or ( D ) by mean fluorescence intensity (MFI) of CD11c, CD14, CD80, CD83, CD86 and CD209 proteins, n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Techniques Used: Incubation, Fluorescence
Figure Legend Snippet: GSK761 enhances the tolerogenic potential of DCs. ( A ) Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. iDCs were washed with PBS and co-cultured with autologous T cells (ratio DCs:T cells 1:10) in presence of 100 ng/mL LPS (for DCs maturation) and Revaxis (for antigen processing and presentation by DCs). The illustration was created with BioRender.com. ( B ) RT 2 Profiler™ PCR Array Human T Helper Cell Differentiation was performed after 24 h of co-culture on T cells purified from DC using CD209 beads, n = 3. ( C ) Relative gene expression of different T-cell markers; Th1 ( TBX21 ), Th2 ( GATA3 ), Th17 ( RORA ) and Treg ( FOXP3 ), n = 3. Statistical significance is indicated as follow: * p < 0.05, ** p < 0.01.
Techniques Used: Incubation, Cell Culture, Cell Differentiation, Co-Culture Assay, Purification, Gene Expression
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