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cd209 dc sign microbead kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd209 dc sign microbead kit
    GSK761 impairs DCs maturation. Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. The cells were then kept unstimulated (iDCs) or stimulated (maturation) with 100 ng/mL LPS for 24 h (mDCs). Differentiation and maturation markers were assessed by FACS. ( A ) Data are illustrated as FACS plots or ( B ) percentage of CD11c + , CD14 + , CD80 + , CD83 + , CD86 + and <t>CD209</t> + cells from total alive cells, n = 3 ( C ) by histogram or ( D ) by mean fluorescence intensity (MFI) of CD11c, CD14, CD80, CD83, CD86 and CD209 proteins, n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Cd209 Dc Sign Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd209+microbead+kit/CD209+(DC-SIGN)+MicroBead+Kit%2C+human/pmc10217447-166-12-16
    Average 91 stars, based on 1 article reviews
    cd209 dc sign microbead kit - by Bioz Stars, 2026-09
    91/100 stars

    Images

    1) Product Images from "The Epigenetic Reader Protein SP140 Regulates Dendritic Cell Activation, Maturation and Tolerogenic Potential"

    Article Title: The Epigenetic Reader Protein SP140 Regulates Dendritic Cell Activation, Maturation and Tolerogenic Potential

    Journal: Current Issues in Molecular Biology

    doi: 10.3390/cimb45050269

    GSK761 impairs DCs maturation. Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. The cells were then kept unstimulated (iDCs) or stimulated (maturation) with 100 ng/mL LPS for 24 h (mDCs). Differentiation and maturation markers were assessed by FACS. ( A ) Data are illustrated as FACS plots or ( B ) percentage of CD11c + , CD14 + , CD80 + , CD83 + , CD86 + and CD209 + cells from total alive cells, n = 3 ( C ) by histogram or ( D ) by mean fluorescence intensity (MFI) of CD11c, CD14, CD80, CD83, CD86 and CD209 proteins, n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Figure Legend Snippet: GSK761 impairs DCs maturation. Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. The cells were then kept unstimulated (iDCs) or stimulated (maturation) with 100 ng/mL LPS for 24 h (mDCs). Differentiation and maturation markers were assessed by FACS. ( A ) Data are illustrated as FACS plots or ( B ) percentage of CD11c + , CD14 + , CD80 + , CD83 + , CD86 + and CD209 + cells from total alive cells, n = 3 ( C ) by histogram or ( D ) by mean fluorescence intensity (MFI) of CD11c, CD14, CD80, CD83, CD86 and CD209 proteins, n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Techniques Used: Incubation, Fluorescence

    GSK761 enhances the tolerogenic potential of DCs. ( A ) Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. iDCs were washed with PBS and co-cultured with autologous T cells (ratio DCs:T cells 1:10) in presence of 100 ng/mL LPS (for DCs maturation) and Revaxis (for antigen processing and presentation by DCs). The illustration was created with BioRender.com. ( B ) RT 2 Profiler™ PCR Array Human T Helper Cell Differentiation was performed after 24 h of co-culture on T cells purified from DC using CD209 beads, n = 3. ( C ) Relative gene expression of different T-cell markers; Th1 ( TBX21 ), Th2 ( GATA3 ), Th17 ( RORA ) and Treg ( FOXP3 ), n = 3. Statistical significance is indicated as follow: * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: GSK761 enhances the tolerogenic potential of DCs. ( A ) Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. iDCs were washed with PBS and co-cultured with autologous T cells (ratio DCs:T cells 1:10) in presence of 100 ng/mL LPS (for DCs maturation) and Revaxis (for antigen processing and presentation by DCs). The illustration was created with BioRender.com. ( B ) RT 2 Profiler™ PCR Array Human T Helper Cell Differentiation was performed after 24 h of co-culture on T cells purified from DC using CD209 beads, n = 3. ( C ) Relative gene expression of different T-cell markers; Th1 ( TBX21 ), Th2 ( GATA3 ), Th17 ( RORA ) and Treg ( FOXP3 ), n = 3. Statistical significance is indicated as follow: * p < 0.05, ** p < 0.01.

    Techniques Used: Incubation, Cell Culture, Cell Differentiation, Co-Culture Assay, Purification, Gene Expression

    Related Articles

    Immunofluorescence:

    Article Title: Impact of oral consumption of heat-treated Bacteroides xylanisolvens DSM 23964 on the level of natural TFα-specific antibodies in human adults
    Article Snippet: It is now generally accepted that the human body exists in close synergy with the gut microbiome and that this cross-talk plays an essential role in human health and disease.. One facet from the many interactions between the microbiome and the immune system is the induction of natural antibodies to commensal bacterial glycans, such as blood group antigens, the alpha-Gal epitope or the Thomsen-Friedenreich (TFα) antigen.. Since we have observed that certain species of the commensal genus Bacteroides express the TFα antigen, we examined whether the oral dietary supplementation of a pasteurised Bacteroides xylanisolvens strain might be able to enhance the level of natural anti-TFα antibodies in healthy adults.

    Microscopy:

    Article Title: Impact of oral consumption of heat-treated Bacteroides xylanisolvens DSM 23964 on the level of natural TFα-specific antibodies in human adults
    Article Snippet: It is now generally accepted that the human body exists in close synergy with the gut microbiome and that this cross-talk plays an essential role in human health and disease.. One facet from the many interactions between the microbiome and the immune system is the induction of natural antibodies to commensal bacterial glycans, such as blood group antigens, the alpha-Gal epitope or the Thomsen-Friedenreich (TFα) antigen.. Since we have observed that certain species of the commensal genus Bacteroides express the TFα antigen, we examined whether the oral dietary supplementation of a pasteurised Bacteroides xylanisolvens strain might be able to enhance the level of natural anti-TFα antibodies in healthy adults.

    other:

    Article Title: The transcription factor MITF is a critical regulator of GPNMB expression in dendritic cells
    Article Snippet: After 7 days of culture, if necessary, DC-SIGN + (CD209 + ) moDC were enriched to > 90% purity prior to qRT-PCR and western blot analyses (CD209 MicroBead Kit, Miltenyi).

    Isolation:

    Article Title: Measles virus suppresses RIG-I-like receptor activation in dendritic cells via DC-SIGN-mediated inhibition of PP1 phosphatases
    Article Snippet: .. DC-SIGN + DCs were isolated by positive selection from BAL cells using the CD209 MicroBead kit (Miltenyi) according to the manufacturer’s instructions. ..

    Article Title: HIV-1 exploits innate signaling by TLR8 and DC-SIGN for productive infection of dendritic cells.
    Article Snippet: 419 Dendritic cells (DCs) are crucial in the induction of antiviral immunity to human immunodeficiency virus type 1 (HIV-1)1.. DCs express pattern-recognition receptors (PRRs) that sense invading pathogens and trigger signaling pathways, which lead to type I interferon responses as well as adaptive immunity2,3.. PRRs such as Toll-like receptors (TLRs), C-type lectins and nucleotide-binding oligomerization domain proteins recognize highly conserved structures expressed on microorganisms called pathogen-associated molecular patterns4–6.

    Article Title: HIV-1 blocks the signaling adaptor MAVS to evade antiviral host defense after sensing of abortive HIV-1 RNA by the host helicase DDX3.
    Article Snippet: Dendritic cells (DCs) are crucial in the induction of adaptive immune responses to infection with HIV-1 (ref. 1); however, DCs do not mount protective immunity against HIV-1 (ref. 2), which is attributed to the ability of HIV-1 to escape immune surveillance.. DCs sense invading viruses through pattern-recognition receptors (PRRs) that trigger type I interferon (IFN) and cytokine responses that lead to intrinsic antiviral defenses and adaptive immunity1.. Type I interferon responses are induced when IFN-α and IFN-β, via autocrine signaling, activate an antiviral program of interferonstimulated genes (ISGs) that counteract virus replication3–5.

    Selection:

    Article Title: Measles virus suppresses RIG-I-like receptor activation in dendritic cells via DC-SIGN-mediated inhibition of PP1 phosphatases
    Article Snippet: .. DC-SIGN + DCs were isolated by positive selection from BAL cells using the CD209 MicroBead kit (Miltenyi) according to the manufacturer’s instructions. ..

    Article Title: HIV-1 exploits innate signaling by TLR8 and DC-SIGN for productive infection of dendritic cells.
    Article Snippet: 419 Dendritic cells (DCs) are crucial in the induction of antiviral immunity to human immunodeficiency virus type 1 (HIV-1)1.. DCs express pattern-recognition receptors (PRRs) that sense invading pathogens and trigger signaling pathways, which lead to type I interferon responses as well as adaptive immunity2,3.. PRRs such as Toll-like receptors (TLRs), C-type lectins and nucleotide-binding oligomerization domain proteins recognize highly conserved structures expressed on microorganisms called pathogen-associated molecular patterns4–6.



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    GSK761 impairs DCs maturation. Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. The cells were then kept unstimulated (iDCs) or stimulated (maturation) with 100 ng/mL LPS for 24 h (mDCs). Differentiation and maturation markers were assessed by FACS. ( A ) Data are illustrated as FACS plots or ( B ) percentage of CD11c + , CD14 + , CD80 + , CD83 + , CD86 + and <t>CD209</t> + cells from total alive cells, n = 3 ( C ) by histogram or ( D ) by mean fluorescence intensity (MFI) of CD11c, CD14, CD80, CD83, CD86 and CD209 proteins, n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    GSK761 impairs DCs maturation. Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. The cells were then kept unstimulated (iDCs) or stimulated (maturation) with 100 ng/mL LPS for 24 h (mDCs). Differentiation and maturation markers were assessed by FACS. ( A ) Data are illustrated as FACS plots or ( B ) percentage of CD11c + , CD14 + , CD80 + , CD83 + , CD86 + and <t>CD209</t> + cells from total alive cells, n = 3 ( C ) by histogram or ( D ) by mean fluorescence intensity (MFI) of CD11c, CD14, CD80, CD83, CD86 and CD209 proteins, n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    Image Search Results


    GSK761 impairs DCs maturation. Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. The cells were then kept unstimulated (iDCs) or stimulated (maturation) with 100 ng/mL LPS for 24 h (mDCs). Differentiation and maturation markers were assessed by FACS. ( A ) Data are illustrated as FACS plots or ( B ) percentage of CD11c + , CD14 + , CD80 + , CD83 + , CD86 + and CD209 + cells from total alive cells, n = 3 ( C ) by histogram or ( D ) by mean fluorescence intensity (MFI) of CD11c, CD14, CD80, CD83, CD86 and CD209 proteins, n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Current Issues in Molecular Biology

    Article Title: The Epigenetic Reader Protein SP140 Regulates Dendritic Cell Activation, Maturation and Tolerogenic Potential

    doi: 10.3390/cimb45050269

    Figure Lengend Snippet: GSK761 impairs DCs maturation. Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. The cells were then kept unstimulated (iDCs) or stimulated (maturation) with 100 ng/mL LPS for 24 h (mDCs). Differentiation and maturation markers were assessed by FACS. ( A ) Data are illustrated as FACS plots or ( B ) percentage of CD11c + , CD14 + , CD80 + , CD83 + , CD86 + and CD209 + cells from total alive cells, n = 3 ( C ) by histogram or ( D ) by mean fluorescence intensity (MFI) of CD11c, CD14, CD80, CD83, CD86 and CD209 proteins, n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: After one day of co-culture, T cells were purified from DCs using CD209 (DC-SIGN) MicroBead Kit (Miltenyi Biotech, Leiden, The Netherlands) according to the manufacturer’s protocol.

    Techniques: Incubation, Fluorescence

    GSK761 enhances the tolerogenic potential of DCs. ( A ) Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. iDCs were washed with PBS and co-cultured with autologous T cells (ratio DCs:T cells 1:10) in presence of 100 ng/mL LPS (for DCs maturation) and Revaxis (for antigen processing and presentation by DCs). The illustration was created with BioRender.com. ( B ) RT 2 Profiler™ PCR Array Human T Helper Cell Differentiation was performed after 24 h of co-culture on T cells purified from DC using CD209 beads, n = 3. ( C ) Relative gene expression of different T-cell markers; Th1 ( TBX21 ), Th2 ( GATA3 ), Th17 ( RORA ) and Treg ( FOXP3 ), n = 3. Statistical significance is indicated as follow: * p < 0.05, ** p < 0.01.

    Journal: Current Issues in Molecular Biology

    Article Title: The Epigenetic Reader Protein SP140 Regulates Dendritic Cell Activation, Maturation and Tolerogenic Potential

    doi: 10.3390/cimb45050269

    Figure Lengend Snippet: GSK761 enhances the tolerogenic potential of DCs. ( A ) Human primary CD14 + monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days in presence of vehicle (0.1% DMSO) or 0.12 µM GSK761. iDCs were washed with PBS and co-cultured with autologous T cells (ratio DCs:T cells 1:10) in presence of 100 ng/mL LPS (for DCs maturation) and Revaxis (for antigen processing and presentation by DCs). The illustration was created with BioRender.com. ( B ) RT 2 Profiler™ PCR Array Human T Helper Cell Differentiation was performed after 24 h of co-culture on T cells purified from DC using CD209 beads, n = 3. ( C ) Relative gene expression of different T-cell markers; Th1 ( TBX21 ), Th2 ( GATA3 ), Th17 ( RORA ) and Treg ( FOXP3 ), n = 3. Statistical significance is indicated as follow: * p < 0.05, ** p < 0.01.

    Article Snippet: After one day of co-culture, T cells were purified from DCs using CD209 (DC-SIGN) MicroBead Kit (Miltenyi Biotech, Leiden, The Netherlands) according to the manufacturer’s protocol.

    Techniques: Incubation, Cell Culture, Cell Differentiation, Co-Culture Assay, Purification, Gene Expression